Chemical, Biochemical, Pharmaceutical Sciences, Analytics, Quality Assurance, Research and Development
Aktualisiert am 09.09.2024
Profil
Freiberufler / Selbstständiger
Remote-Arbeit
Verfügbar ab: 01.10.2024
Verfügbar zu: 100%
davon vor Ort: 100%
Laboratory Quality Control
Forschung
HPLC
R&D
chemie
biochemie
English
German
French
Italian
Polish

Einsatzorte

Einsatzorte

Heidelberg (+20km) Mannheim (+20km) Ludwigshafen am Rhein (+20km)
Deutschland
möglich

Projekte

Projekte

2 years 1 month
2022-09 - 2024-09

DISRUPT

Postdoctoral Researcher
Postdoctoral Researcher

Towards the Targeting of Self-Assembling Proteins in Drug Design, Laboratory for the Analysis of Medicines GMP, CIRM, University of Liège, Belgium,


Key achievements: 

Optimization of SEC separation of native, mutated and truncated protein oligomers and reducing proteincolumn interactions


Tasks:

  • SEC-MALS analysis, GMP qualification and maintenance of laboratory instruments, project management, preparation of scientific articles

2 years 3 months
2020-07 - 2022-09

Application of N-[2-thioethyl]glycine analogues

Principal Investigator in the Grant Preludium 18
Principal Investigator in the Grant Preludium 18
  • Application of N-[2-thioethyl]glycine analogues in orthogonal cleavage of peptides from solid support
Polish National Science Centre
5 years
2017-10 - 2022-09

Development of N-[2-thioethyl]glycine cryptothioester

Ph.D. student
Ph.D. student
Development of N-[2-thioethyl]glycine cryptothioester for orthogonal peptide
cleavage, thioesterification and cyclative Native Chemical Ligation (NCL)
  • project management, organic synthesis, solid-phase peptide synthesis (SPPS) and modification, NCL, HPLC, UV-vis, NMR, LC-ESI-MS and MS/MS analysis, preparation of scientific articles and grant projects, supervision of master?s and bachelor?s projects, Teaching courses: Organic Chemistry, Bioorganic Chemistry
Peptide Engineering Group, Faculty of Chemistry, University of Wroclaw, Poland
1 month
2022-05 - 2022-05

Internship

financed by the SSHN Campus France program, French Government Scholarship (BGF)

  • Synthesis of the transactivation domain (TAD) of p53 protein with N-[2-thioethyl]glycine thioester precursor

  • Automated SPPS and NCL, protein synthesis and purification, LC-MS and UV-vis analysis

Biosystem Chemistry Group, High School of Biotechnology, University of Strasbourg, France
1 month
2021-06 - 2021-06

Internship

Kinetic studies on new N,S-acyl shift devices for peptide thioesterification

  • Organic Synthesis
  • Automated SPPS
  • thioesterificaton and NCL
  • UV-vis
  • NMR, LC-MS analysis

Protein Synthesis and Biorthogonal Chemistry Research Group,Centre of Biomolecular Physics, CNRS-Orléans, France
9 months
2017-01 - 2017-09

Erasmus+ Research Project and Traineeship

Development of solid support for selective capturing of glycated and glycosylated peptides and their LC-MS identification
  • SPPS
  • selective capturing of sugar-modified peptides
  • UV-vis
  • LC-MS analysis
French-Italian Interdepartmental Laboratory of Peptide and Protein Chemistry and Biology, University of Florence, Italy
1 year 8 months
2015-05 - 2016-12

synthesis and fragmentation analysis of peptide conjugates

Investigator in the Grant Opus 5
Investigator in the Grant Opus 5
  • The synthesis and fragmentation analysis of peptide conjugates with betaine analogues for the investigation of new isobaric tags for quantitative proteomics
Polish National Science Centre
1 year 3 months
2015-07 - 2016-09

Safirinium analogues

Investigator
Investigator
  • The application of Safirinium analogues as potential ionization enhancers in proteomics research
Grant from Wroclaw Centre of Biotechnology

Aus- und Weiterbildung

Aus- und Weiterbildung

1 month
2024-04 - 2024-04

Scientific Writing

Elsevier Researcher Academy (On-line)
Elsevier Researcher Academy (On-line)
1 month
2024-04 - 2024-04

Prague Computational Mass Spectrometry Workshop

IOCB Prague, Czechia
IOCB Prague, Czechia
1 month
2023-07 - 2023-07

Protein Stabilization: Design, Experiments and Assessment (ProteSta)

MOSBRI Advanced Level Course, Institute BIFI ? University of Zaragoza (BIFI - LACRIMA)
MOSBRI Advanced Level Course
Institute BIFI ? University of Zaragoza (BIFI - LACRIMA)
1 month
2023-05 - 2023-05

International Post-graduate Course: Protein quality control

Avans University of Applied Sciences, Research Group Analysis Techniques in Life Science, Breda, The Netherlands
Avans University of Applied Sciences, Research Group Analysis Techniques in Life Science, Breda, The Netherlands
1 month
2022-11 - 2022-11

SEC-MALS Training

Light Scattering University, Wyatt Technology, Dernbach
Light Scattering University, Wyatt Technology, Dernbach
4 years 9 months
2017-10 - 2022-06

Studies in Chemical Sciences

Doctoral, University of Wroclaw, Faculty of Chemistry, Poland
Doctoral
University of Wroclaw, Faculty of Chemistry, Poland
  • Ph.D. thesis: on request
1 month
2018-10 - 2018-10

The fundamental training for LCMS-9030, Nexera Mikros, MALDI-8020 and iMScope

Shimadzu Corporation, Kyoto, Japan
Shimadzu Corporation, Kyoto, Japan
1 month
2018-09 - 2018-09

LCMS-IT-TOF Operator Training

Shimadzu Europa GmbH, Wroc?aw, Poland
Shimadzu Europa GmbH, Wroc?aw, Poland
1 month
2018-04 - 2018-04

Academy of Analytical Chemistry

Mass spectrometry (LC-MS) in the analysis of lowmass analytes, Shim-POL, Jachranka, Poland
Mass spectrometry (LC-MS) in the analysis of lowmass analytes
Shim-POL, Jachranka, Poland
4 years 9 months
2012-10 - 2017-06

Studies in Biological Chemistry

Bachelor?s and Master?s, University of Wroclaw, Faculty of Chemistry, Poland
Bachelor?s and Master?s
University of Wroclaw, Faculty of Chemistry, Poland

Key achievements: 

  • Synthesis and examination of ionization tags containing the Safirnium and 5-azoniaspiro[4.4]nonyl systems for improved detection by MS


Tasks:

  • development of ionization and isotopic peptide tags for proteomics, SPPS, HPLC, LC-MS and MS/MS analysis

1 month
2016-05 - 2016-05

LCMS-8040, GCMS-QP2010 Ultra

SHIM-POL, Wroc?aw, Poland
SHIM-POL, Wroc?aw, Poland

Kompetenzen

Kompetenzen

Top-Skills

Laboratory Quality Control Forschung HPLC R&D chemie biochemie

Produkte / Standards / Erfahrungen / Methoden

Profile:

I am currently a Postdoctoral Researcher at the University of Liège where I optimized separative techniques for assessing protein oligomerization, by SEC-MALS and native MS. I completed doctoral studies at the University of Wroclaw, where I developed a new synthetic way for peptide cyclization by Native Chemical Ligation. I am experienced especially in HPLC, MS, peptide synthesis and modification, and I am highly motivated to learn new techniques and explore protein science.


IT

  • LabSolutions (Shimadzu)
  • OpenLab (Agilent)
  • Compass Data Analysis 4.0 (Bruker)
  • Astra 8 (Wyatt)
  • MzMine
  • MestReNova
  • ChemSketch
  • ChemDraw
  • Microsoft Office
  • IOS


Instrumental Skills:

  • NMR, UV-VIS, MALS
  • HPLC, LC-MS, LC-UV-MS
  • HR-MS:
    • ESI-FT-ICR
    • ESI-IT-TOF
    • ESI-qTOF
    • ESI-QQQ
    • ESI-IMS
    • ?MS/MS, MSn (CID)


Skills:

  • Project Management
  • Analytical Thinking
  • Strategic Planning
  • Ability to Work Under Pressure
  • Communication Skills
  • Adaptability
  • Ability to Work in a Team
  • Computer Skills


Experimental skills

Advanced organic synthesis,

Solid phase peptide synthesis:

  • manual and automated,
  • Peptide/protein modification


Separation techniques:

  • HPLC
  • RP
  • SEC
  • SPE
  • flash chromatography

Einsatzorte

Einsatzorte

Heidelberg (+20km) Mannheim (+20km) Ludwigshafen am Rhein (+20km)
Deutschland
möglich

Projekte

Projekte

2 years 1 month
2022-09 - 2024-09

DISRUPT

Postdoctoral Researcher
Postdoctoral Researcher

Towards the Targeting of Self-Assembling Proteins in Drug Design, Laboratory for the Analysis of Medicines GMP, CIRM, University of Liège, Belgium,


Key achievements: 

Optimization of SEC separation of native, mutated and truncated protein oligomers and reducing proteincolumn interactions


Tasks:

  • SEC-MALS analysis, GMP qualification and maintenance of laboratory instruments, project management, preparation of scientific articles

2 years 3 months
2020-07 - 2022-09

Application of N-[2-thioethyl]glycine analogues

Principal Investigator in the Grant Preludium 18
Principal Investigator in the Grant Preludium 18
  • Application of N-[2-thioethyl]glycine analogues in orthogonal cleavage of peptides from solid support
Polish National Science Centre
5 years
2017-10 - 2022-09

Development of N-[2-thioethyl]glycine cryptothioester

Ph.D. student
Ph.D. student
Development of N-[2-thioethyl]glycine cryptothioester for orthogonal peptide
cleavage, thioesterification and cyclative Native Chemical Ligation (NCL)
  • project management, organic synthesis, solid-phase peptide synthesis (SPPS) and modification, NCL, HPLC, UV-vis, NMR, LC-ESI-MS and MS/MS analysis, preparation of scientific articles and grant projects, supervision of master?s and bachelor?s projects, Teaching courses: Organic Chemistry, Bioorganic Chemistry
Peptide Engineering Group, Faculty of Chemistry, University of Wroclaw, Poland
1 month
2022-05 - 2022-05

Internship

financed by the SSHN Campus France program, French Government Scholarship (BGF)

  • Synthesis of the transactivation domain (TAD) of p53 protein with N-[2-thioethyl]glycine thioester precursor

  • Automated SPPS and NCL, protein synthesis and purification, LC-MS and UV-vis analysis

Biosystem Chemistry Group, High School of Biotechnology, University of Strasbourg, France
1 month
2021-06 - 2021-06

Internship

Kinetic studies on new N,S-acyl shift devices for peptide thioesterification

  • Organic Synthesis
  • Automated SPPS
  • thioesterificaton and NCL
  • UV-vis
  • NMR, LC-MS analysis

Protein Synthesis and Biorthogonal Chemistry Research Group,Centre of Biomolecular Physics, CNRS-Orléans, France
9 months
2017-01 - 2017-09

Erasmus+ Research Project and Traineeship

Development of solid support for selective capturing of glycated and glycosylated peptides and their LC-MS identification
  • SPPS
  • selective capturing of sugar-modified peptides
  • UV-vis
  • LC-MS analysis
French-Italian Interdepartmental Laboratory of Peptide and Protein Chemistry and Biology, University of Florence, Italy
1 year 8 months
2015-05 - 2016-12

synthesis and fragmentation analysis of peptide conjugates

Investigator in the Grant Opus 5
Investigator in the Grant Opus 5
  • The synthesis and fragmentation analysis of peptide conjugates with betaine analogues for the investigation of new isobaric tags for quantitative proteomics
Polish National Science Centre
1 year 3 months
2015-07 - 2016-09

Safirinium analogues

Investigator
Investigator
  • The application of Safirinium analogues as potential ionization enhancers in proteomics research
Grant from Wroclaw Centre of Biotechnology

Aus- und Weiterbildung

Aus- und Weiterbildung

1 month
2024-04 - 2024-04

Scientific Writing

Elsevier Researcher Academy (On-line)
Elsevier Researcher Academy (On-line)
1 month
2024-04 - 2024-04

Prague Computational Mass Spectrometry Workshop

IOCB Prague, Czechia
IOCB Prague, Czechia
1 month
2023-07 - 2023-07

Protein Stabilization: Design, Experiments and Assessment (ProteSta)

MOSBRI Advanced Level Course, Institute BIFI ? University of Zaragoza (BIFI - LACRIMA)
MOSBRI Advanced Level Course
Institute BIFI ? University of Zaragoza (BIFI - LACRIMA)
1 month
2023-05 - 2023-05

International Post-graduate Course: Protein quality control

Avans University of Applied Sciences, Research Group Analysis Techniques in Life Science, Breda, The Netherlands
Avans University of Applied Sciences, Research Group Analysis Techniques in Life Science, Breda, The Netherlands
1 month
2022-11 - 2022-11

SEC-MALS Training

Light Scattering University, Wyatt Technology, Dernbach
Light Scattering University, Wyatt Technology, Dernbach
4 years 9 months
2017-10 - 2022-06

Studies in Chemical Sciences

Doctoral, University of Wroclaw, Faculty of Chemistry, Poland
Doctoral
University of Wroclaw, Faculty of Chemistry, Poland
  • Ph.D. thesis: on request
1 month
2018-10 - 2018-10

The fundamental training for LCMS-9030, Nexera Mikros, MALDI-8020 and iMScope

Shimadzu Corporation, Kyoto, Japan
Shimadzu Corporation, Kyoto, Japan
1 month
2018-09 - 2018-09

LCMS-IT-TOF Operator Training

Shimadzu Europa GmbH, Wroc?aw, Poland
Shimadzu Europa GmbH, Wroc?aw, Poland
1 month
2018-04 - 2018-04

Academy of Analytical Chemistry

Mass spectrometry (LC-MS) in the analysis of lowmass analytes, Shim-POL, Jachranka, Poland
Mass spectrometry (LC-MS) in the analysis of lowmass analytes
Shim-POL, Jachranka, Poland
4 years 9 months
2012-10 - 2017-06

Studies in Biological Chemistry

Bachelor?s and Master?s, University of Wroclaw, Faculty of Chemistry, Poland
Bachelor?s and Master?s
University of Wroclaw, Faculty of Chemistry, Poland

Key achievements: 

  • Synthesis and examination of ionization tags containing the Safirnium and 5-azoniaspiro[4.4]nonyl systems for improved detection by MS


Tasks:

  • development of ionization and isotopic peptide tags for proteomics, SPPS, HPLC, LC-MS and MS/MS analysis

1 month
2016-05 - 2016-05

LCMS-8040, GCMS-QP2010 Ultra

SHIM-POL, Wroc?aw, Poland
SHIM-POL, Wroc?aw, Poland

Kompetenzen

Kompetenzen

Top-Skills

Laboratory Quality Control Forschung HPLC R&D chemie biochemie

Produkte / Standards / Erfahrungen / Methoden

Profile:

I am currently a Postdoctoral Researcher at the University of Liège where I optimized separative techniques for assessing protein oligomerization, by SEC-MALS and native MS. I completed doctoral studies at the University of Wroclaw, where I developed a new synthetic way for peptide cyclization by Native Chemical Ligation. I am experienced especially in HPLC, MS, peptide synthesis and modification, and I am highly motivated to learn new techniques and explore protein science.


IT

  • LabSolutions (Shimadzu)
  • OpenLab (Agilent)
  • Compass Data Analysis 4.0 (Bruker)
  • Astra 8 (Wyatt)
  • MzMine
  • MestReNova
  • ChemSketch
  • ChemDraw
  • Microsoft Office
  • IOS


Instrumental Skills:

  • NMR, UV-VIS, MALS
  • HPLC, LC-MS, LC-UV-MS
  • HR-MS:
    • ESI-FT-ICR
    • ESI-IT-TOF
    • ESI-qTOF
    • ESI-QQQ
    • ESI-IMS
    • ?MS/MS, MSn (CID)


Skills:

  • Project Management
  • Analytical Thinking
  • Strategic Planning
  • Ability to Work Under Pressure
  • Communication Skills
  • Adaptability
  • Ability to Work in a Team
  • Computer Skills


Experimental skills

Advanced organic synthesis,

Solid phase peptide synthesis:

  • manual and automated,
  • Peptide/protein modification


Separation techniques:

  • HPLC
  • RP
  • SEC
  • SPE
  • flash chromatography

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